Document

The quantitative determination of the adulterated dexamethasone and prednisolone in traditional drug was developed by using high performance liquid chromatography (HPLC) with reversed phase C8 column (Hypersil BDS) and a mixture of acetonitrile and purified water as a mobile phase. The sample solution was cleaned up by solid phase extraction (SPE). The method validation data showed good linearity at the concentration range of 250-12,500 μg/L with the correlation coefficient of 0.9987 and 0.9988 for dexamethasone and prednisolone, respectively. Accuracy and precision were performed by adding 500, 6,000 and 12,000 μg/L of each of dexamethasone and prednisolone to sample matrix. The percentages of recovery were 100.30-104.53 and 100.02-102.18 for dexamethasone and prednisolone, respectively. The relative standard deviations of dexamethasone and prednisolone were 0.04-2.01 and 0.15-2.47, respectively. The limit of quantitation of dexamethasone and prednisolone are 250.14 and 249.30 μg/L, respectively. Therefore, the developed HPLC method is suitable for surveillance of the dexamethasone and prednisolone adulterants in traditional herbal medicines.

https://he02.tci-thaijo.org/index.php/dmsc/article/view/242649