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According to The Narcotics Control Act of 2519 B.E., anybody who is detected morphine in urine at the 300 ng/ml cutoff threshold, they will be reported as the drugs user. However, opium, codeine, heroin, or morphine user can meet morphine in urine. However, 6MAM can use to identify using morphine and Codeine-morphine ratio above 1 use to identify using codeine. Thus, these sunstances can derive the sources of morphine. The aim of the present work was to derive the sources of morphine in body fluids and quantification of morphine correctly using on-line solid phase extraction-liquid chromatography-tandem mass spectrometry. morphine-d3 and 6MAM-d3 are used as internal standard. Extraction by online SPE. Separation was performed on an ion-exchange column and detected by tandem mass spectrometry with electron spray ionization, positive mode. The method was validated according to the FDA guidance. Limit of 6MAM detection is 1 ng /ml. The linear range of codeine and morphine were obtained from 100-2000 ng/mL. This developed method was successfully using for quantification of morphine in human urine and its source. Can apply the method for classification of drug user in routine job of department of medical science

Keywords : Morphine, Codeine, Heroine, 6MAM, urine